Thinprep pap test kit with preservcyt solution vial obtain directly from the allina cytology lab at abbott northwestern hospital by calling 6128634678, option 2 if there are none on hand. Introduced in the 1940s, pap testing has been the single greatest contributor to the overall decline in cervical cancer. Only 1 aliquot may be removed from preservcyt sample vial prior to performing the thinprep pap test, regardless of the volume of the aliquot maximum aliquot volume3 ml. The cobas hpv test is not intended for use with samples other than those collected by a clinician using an endocervical brush or spatula and placed in the thinprep pap test preservcyt solution. All unsatisfactory thinprep samples were reprocessed using a glacial acetic acid wash. Of the 124 abnormal tp samples, after reprocessing, surgical pathology follow.
Gyenocologic pap test collection procedure medical center. Within the last decade, surepath and thinprep both liquidbased cytology lbc tests have replaced conventional cytology cc as primary test method in cervical cancer screening programs of multiple countries. It can also reveal changes in your cervical cells that may turn into cancer later. Immediate, fire hazard methanol is listed on the 3 toxic pollutant reporting list. A modified pap test technique designed to reduce some of the technical problems inherent in the traditional type of pap smear in the traditional pap test, cell samples are obtained from the vagina, cervix, and cervical canal and spread on a glass slide. Insert the central bristles of the broom into the endocervical canal deep enough to allow the shorter bristles to fully contact the.
You need a repeat pap test and hpv test 12 months after that. Original thinpreppreservcyt collection vial is required for testing. Product name thinprep preservcyt solution safety data sheet number 85093001 recommended use of the chemical and restrictions on use specific uses a methanol based, buffered preservative solution used to support cells during transport and slide preparation recommended use in vitro diagnostic details of the supplier of the safety data sheet. Conclusions regarding the thinprep are difficult to make due to the complexity of cervical cancer screening and the lack of adequate outcomebased data. Hologic thinprep preservcyt solution material safety data. The thinprep pap test is the first real improvement to the pap smear since it was introduced 50 years ago. It is important to note that lubricant increases the risk of contaminating or obscuring the. Some physician training in proper collection techniques is also needed. The cells are then collected on a thinprep pap test filter specifically designed to collect diagnostic cells.
Pap test, has led to a significant reduction in the incidence of and mortality from cervical cancer1. Ensure the entire plastic seal is removed from the lid of the vial and discarded. Lubricant use during pap test collection for the thinprep pap dear colleague. Therefore, when collecting specimens, it is important to use appropriate devices and techniques, such as avoiding the use. You need to have a pap test and colposcopy 46 months after treatment. For optimal interpretation, pap smears should be collected near the middle of the menstrual cycle.
The study period unsatisfactory rate was compared with that for the previous 12 months. Thinprep pap test instructions for direct to vial specimen collection quick reference guide obtain an adequate sample from the transformation zone of the cervix using a cervical sampler broomlike device. These results demonstrate that preservcyt transport medium is a suitable transport medium for detection of c. Satisfactory thinprep pap test specimen patient information the patient should be tested two weeks after the first day of her last menstrual period, and definitely not when she is menstruating. In general, the guidelines state that it is important to obtain a specimen that is not obscured by blood, mucus, inflammatory exudate or lubricant. As with any laboratory test, both false negative and false positive results may occur. Therefore, when collecting specimens for thinprep pap testing, it is important to use appropriate devices and techniques and to avoid the use of lubricants. More severe of pap test register result and masked histology rereading as reference standard. Comparing surepath, thinprep, and conventional cytology as. From a single specimen collected in the thinprep test vial, doctors can screen for cervical disease and hpv, as well as common sexually transmitted infections stis confidently and accurately.
Is the thinprep better than conventional pap smear at. The thinprep pap test is the only liquidbased pap test fdaapprovedcleared for hpv, chlamydiagonorrhea and trichomonas testing out of the same vial. Hologic thinprep 5000 operators manual pdf download. The preservcyt solution thinprep vial will be processed as a pap first, then additional tests will follow. To arrange training, contact laboratory customer service at 924labs. The following guidelines are referenced from clsi document gp15a31 and are recommended in the collection process for obtaining a thinprep pap test. The results from this study showed a detection rate of 51120,917 for the conventional pap smear versus 39910. Lubricant use during pap test collection for the thinprep pap. Oct 12, 2015 within the last decade, surepath and thinprep both liquidbased cytology lbc tests have replaced conventional cytology cc as primary test method in cervical cancer screening programs of multiple countries. Quick reference guide thinprep pap test plastic spatula and endocervical brush protocol prepare all equipment before starting the procedure.
Teilbericht fruherkennung des zervixkarzinoms, hier. Therefore, when collecting specimens, it is important to use appropriate devices and techniques, such as avoiding the use of lubricants, or if they are used, using them sparingly. The patient should refrain from intercourse 48 hours prior to the exam. The thinprep pap test is the first real improvement to the conventional pap smear in 50 years. Test samples for the thinprep pap test are collected the same way as those for the conventional pap test. If diagnostic please indicate patients signs, symptoms orhistory. This new test gives a new level of confidence in the reliability of your results. Original thinprep preservcyt collection vial is required for testing. Be in control of your own body and ask your doctornurse for a thinprep pap test today. The patient should be tested 2 weeks after the first day of her. The purpose of this study was to compare tp performance and. Positive results should be confirmed with additional studies as clinically indicated.
Background the thinprep pap test tp, a liquidbased cervical cytology preparation, was approved for use in the u. Thinprep sample collection instructions national reference. Include date of birth, social security number or other identification number, previous malignancy, drug therapy, radiation therapy, last menstrual period lmp, postmenopausal patient pmp, surgery including surgical biopsies, exogenous hormones, abnormal vaginal bleeding, abnormal pap results, iud, and all other pertinent clinical information on the cytology test request form. Gynecologic pap test, liquidbased preparation labcorp. Even though the tppt reduces obscuring blood, clinical studies have demonstrated that excessive amounts of blood. Thinprep pap test wisconsin state laboratory of hygiene. The thinprep pap test dsc clinic department of sti. Reprocessing unsatisfactory thinprep papanicolaou test. However, the specimen is placed in a preservative solution rather than on a slide. It combines computer imaging technology to identify microscopic fields of diagnostic interest with automated stage movement of a microscope in order to locate these fields. Pdf thinprep pap test promotes detection of glandular lesions of. The pap test is a valuable screening test for cervical cancer and its precursors. To reduce misclassification the two histological assessments were combined, using the more severe of the two results as the reference.
Hpvnegative cancers of the cervix do occur in rare circumstances. Pap smear specimen collection using thinprep pap test method print new copies from intranet last approved date. Labcorp test details for gynecologic pap test, liquidbased preparation and chlamydiagonococcus, naa with reflex to human papillomavirus hpv highrisk dna detection when ascu. In addition, an instructional video on thinprep pap test collection is available from the cytology laboratory 924.
Thinprep prepared slides by hologic or by organizations or individuals designated by hologic. Accuracy of reading liquid based cytology slides using the. Finally, although triennial screening with thinprep is a costeffective approach for significantly decreasing the incidence of cervical disease, ultimately, it will take not only improving the sensitivity of the pap test, but also increasing participation in screening programs, especially for highrisk women, in order to continue to reduce the. Lubricants may interfere with the processing and interpretation of the specimen. Be in control of your own body and ask your doctornurse for a thinprep pap. These include preservcyt solution vials, thinprep pap test filters, and thinprep microscope slides. You need another pap test and hpv test 12 months after treatment. Then, the 1996 launch of the hologic thinprep pap test propelled screening success to new heights. Thinprep sample collection instructions specimen collection is a key factor in obtaining adequate and representative samples for analysis. View and download hologic thinprep operators manual online. Preservcyt transport medium used for the thinprep pap test is. A pap test, also called a pap smear, is an exam a doctor uses to test for cervical cancer in women. Sensitivity and specificity of conventional pap test, liquidbased cytology and.
A positive highrisk hpv result associated with an unsatisfactory thinprep pap test result identified patients at risk for diagnosis of an undetected squamous intraepithelialcervical. The thinprep pap test was the first liquidbased cytology option in cervical cancer screening. Dyke large spills with absorbent with an inert material e. Thinprep pap test specimen collection specimen collection is a key factor in obtaining adequate and representative samples for analysis. Preservcyt transport medium used for the thinprep pap test. If both of these tests are normal, you can return to twoyearly pap tests. Diagnostic accuracy of conventional pap test, liquidbased. Quick reference guide thinprep pap test cervical sampler broom protocol prepare all equipment before starting the procedure. On occasion we are asked to provide information concerning the use of lubricants when collecting a pap test. Pap smear specimen collection using thinprep pap test method. Thinprep pap test instructions for direct to vial specimen collection liquid based cytology collection. The thinprep pap test may enable gynecologists to monitor for both cervical lesions and c. This prospective study analyzed the potential benefits of a laboratory procedure to reprocess unsatisfactory thinprep pap tests cytyc, boxborough, ma.
Insert the brush into the endocervical canal until only the bottommost fibers are exposed. Apr 20, 2020 test samples for the thinprep pap test are collected the same way as those for the conventional pap test. The following guidelines are referenced from clsi document gpi 5. The cobas hpv test is not intended for women who have undergone hysterectomy. Supplies used in the thinprep 2000 system are those designed and supplied by hologic specifically for the thinprep 2000 system. The thinprep 2000 processor constantly monitors the. The remaining 93 samples had either insufficient tissue for pathologic assessment or inadequate follow. Thinprep versus the conventional papanicolaou test.
When using the thinprep method, a gynecologic specimen is collected using a brush and plastic spatula as a cervical sampling system. Through improved sample preparation resulting in fewer recalls, increased disease detection allowing greater confidence in diagnosis and the ability to run further test as required the thinprep. Pdf the objectives of this study were to evaluate 1 the detection rate of atypical glandular cells of undetermined significance of endocervical. Pdf the unsatisfactory thinprep pap test joel bentz. Thinprep pap testquick reference guide broomlike device protocol title. Gyenocologic pap test collection procedure medical. The record in the pap test register and result of masked rereading of histology agreed in 81% of cases. Thinprep preservcyt solution material safety data sheet msds avoid dispersal of spilled material, runoff and contact with soil, waterways, drains and sewers. Training bulletin hologic the science of sure specimen collection the detection of cervical cancer and its precursors as well as other gynecologic abnormalities is the primary purpose of obtaining a cervical cell sample. The thinprep pap test requires unique specimen collectors and a special collection fluid. The thinprep 5000 autoloader system maximizes laboratory efficiency while offering even more walkaway freedom with up to 8 hours of handsfree processing of thinprep pap test, thinprep nongyn and thinprep urocyte samples. One small specimen transport bag with outer document pocket. Slowly rotate the brush 1 4 to 1 2 turn in one direction.
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